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Isolation of a vascular cell wall-specific monoclonal antibody recognizing a cell polarity by using a phage display subtraction method

机译：血管细胞壁特异性单克隆抗体的分离通过噬菌体展示识别细胞极性减法

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Using a strategy consisting of (i) the isolation of cell walls from synchronously differentiating cells of Zinnia, (ii) the generation of mAbs with an antibody phage display method, and (iii) screening with a subtraction method, we isolated mAbs recognizing vascular development-specific cell wall components without prior antigen identification. One of the isolated mAbs, designated CN 8, recognized a cell wall component contained in the hemicellulosic fraction. Immunohistochemical analyses showed that the CN 8 epitope was localized to the cell wall of immature tracheary elements and xylem parenchyma cells. In immature tracheary elements, the CN 8 epitope had a polarized localization pattern regardless of whether the cells are formed as parts of vessels in situ or as single tracheary elements in vitro, suggesting that cell polarity autonomously formed on the cell wall may function in tracheary element differentiation.

机译：使用以下策略组成：（i）从百日草同步分化细胞中分离细胞壁，（ii）用抗体噬菌体展示方法生成mAb，以及（iii）用减法筛选，我们分离出识别血管发育的mAb特异性的细胞壁成分，无需事先进行抗原鉴定。分离的单克隆抗体之一，命名为CN 8，识别了半纤维素级分中所含的细胞壁成分。免疫组织化学分析表明，CN 8表位位于未成熟气管元件和木质部实质细胞的细胞壁。在未成熟的气管元件中，CN 8表位具有极化的定位模式，无论细胞是在原位形成为血管的一部分还是在体外形成为单个气管元件，提示在细胞壁上自主形成的细胞极性可能在气管元件中起作用差异化。

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Shinohara, Naoki; Demura, Taku; Fukuda, Hiroo;
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年度 2000
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1. Isolation of a vascular cell wall-specific monoclonal antibody recognizing a cell polarity by using a phage display subtraction method [J] . Naoki Shinohara, Taku Demura, Hiroo Fukuda Proceedings of the National Academy of Sciences of the United States of America . 2000,第6期

机译：通过噬菌体展示扣除法分离识别细胞极性的血管细胞壁特异性单克隆抗体
2. Isolation of monoclonal antibodies recognizing rare and dominant epitopes in plant vascular cell walls by phage display subtraction. [J] . Shinohara N iol.s.u-tokyo.ac.jp, Fukuda H Journal of Immunological Methods . 2002,第1a2期

机译：通过噬菌体展示减法分离识别植物血管细胞壁中稀有和显性表位的单克隆抗体。
3. Isolation of cell surface-specific human monoclonal antibodies using phage display and magnetically-activated cell sorting: applications in immunohematology. [J] . Siegel DL, Chang TY, Russell SL, Journal of Immunological Methods . 1997,第1a2期

机译：使用噬菌体展示和磁激活细胞分选技术分离细胞表面特异性人单克隆抗体：在免疫血液学中的应用。
4. ISOLATION OF MONOCLONAL ANTIBODIES RECOGNIZING XYLEM CELL WALL COMPONENTS BY USING A PHAGE DISPLAY SUBTRACTION METHOD [C] . Naoki Shinohara, Taku Demura, Hiroo Fukuda International Wood Biotechnology Symposium (IWBS) Mar 14-17, 2001, Narita, Chiba, Japan . 2001

机译：噬菌体色谱分离法分离识别木聚糖壁成分的单克隆抗体。
5. The Isolation of gp41 Specific Monoclonal Antibodies from the Cervical IgA Repertoire of Highly Exposed Persistently Seronegative (HEPS) Commercial Sex Workers from Nairobi, Kenya using Mammalian Cell Display. [D] . Gaudet, Ryan G. 2010

机译：使用哺乳动物细胞展示从肯尼亚内罗毕的高度暴露的持久性血清阴性（HEPS）商业性工作者的宫颈IgA库中分离出gp41特异性单克隆抗体。
6. Isolation of a vascular cell wall-specific monoclonal antibody recognizing a cell polarity by using a phage display subtraction method [O] . Naoki Shinohara, Taku Demura, Hiroo Fukuda 2000

机译：血管细胞壁特异性单克隆抗体的分离通过噬菌体展示识别细胞极性减法
7. Phage display derived human monoclonal antibodies isolated by binding to the surface of live primary breast cancer cells recognize GRP78 [O] . Jakobsen Charlotte G, Rasmussen Nicolaj, Laenkholm Anne-Vibeke, 2007

机译：噬菌体展示衍生的人单克隆抗体通过与活的原发性乳腺癌细胞表面结合而分离，可识别GRP78

Isolation of a vascular cell wall-specific monoclonal antibody recognizing a cell polarity by using a phage display subtraction method

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